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Research Shows In Situ Monitoring Improves Bioprocess Control in New White Paper by Petter Taerbe





Article Author Biography
Research Shows In Situ Monitoring Improves Bioprocess Control in New White Paper by
Article Posted: 05/06/2014
Article Views: 105
Articles Written: 80
Word Count: 447
Article Votes: 0
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Research Shows In Situ Monitoring Improves Bioprocess Control in New White Paper


 
Business

A new white paper from METTLER TOLEDO explores how inline monitoring techniques and simplified spectroscopy calibration can help maximize yield, batch-to-batch reproducibility, and economics of bioproducts as demand for them continues to increase.

METTLER TOLEDO is pleased to present a new installment in its white paper series on in situ spectroscopy applications. The paper, entitled "Understand and Control Bioprocesses: In Situ Analysis for Fermentation Optimization," reviews three studies that developed simple, validated in situ spectroscopy calibration models for accurate analyte monitoring in fermentation processes resulting in greater process control and enhanced process economics.

Inline monitoring of key process analytes such as carbon source, nitrogen source, and specific undesired metabolites can have significant impact on process understanding. The information obtained, such as optimal harvest time, can offer manufacturers significant economic rewards throughenhanced productivity and quality. Additionally, inline technologies eliminate sample preparation, are non-invasive to the process, and provide real-time information on the ongoing process inside the bioreactor.

Inline monitoring has historically been seen as arduous because of the number of standards required for calibration. However, the three studies described in the paper help put this idea to rest.

Case Study 1 uses batch-fed Pencilliumchrysogentum to quantify development of both Penicillin V and its precursor for enhanced yield. Case Study 2 employsa single-spectra library for simple FTIR spectroscopy calibration, gaining a 6-fold decrease in the number of standards required. Case Study 3 explores FTIR and measurement of fermentation substrate concentrations. In each case study, effective strategies were created to develop reliable, robust calibration models, enabling real-time inline monitoring of the fermentation processes.

Results included better process reproducibility, yield, and time-savings when compared to either older FTIR spectroscopy monitoring or traditional offline sampling. For information on the case studiesas well as enabling ReactIR technology, download the white paper at http://www.mt.com/wp-bioprocesses?crel=ePR_PP_EN.

About Mettler Toledo:
METTLER TOLEDO specializes in the area of precision instruments for professional use. METTLER TOLEDO laboratory instruments are used in research, scientific, drug discovery, and quality control labs, amongst many others in the pharmaceutical, chemical, food and cosmetics industries. METTLER TOLEDO comprehensive industrial solutions cover the various steps in a host of manufacturing processes at many of the same customers that laboratory serves. Solutions range from receiving raw materials through various manufacturing processes, in-line process control and end-of-line packaging control, to logistics and shipping. Increasingly, these solutions are fully integrated into the customer's IT environment, helping automate their workflows. In food retail, our offering for fresh food management ranges from receiving and pre-packaging, to in-store solutions for self-service departments, deli counters, and checkout terminals.

Contact information:
Mettler-Toledo International Inc.
Im Langacher, Zip Code 8606
Greifensee, Switzerland
E-Mail : ePublicRelations@mt.com
Phone : +41-44-944 33 85
Website : www.mt.com

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