|
|
 |
|
|
ELISA Kit by Johnny James
|
|
|
ELISA Kit by JOHNNY JAMES
|
|
| Article Posted: 12/28/2012 |
| Article Views: 486 |
| Articles Written: 1 |
| Word Count: 91 |
| Article Votes: 0 |
|
ELISA Kit |
|
|
|
|
|
Business,Link Popularity,MLM
|
|
|
ELISA Kit Enzyme linked immunosorbent assay, enzyme immunoassay is the most widely used technology. The basic method is will known antigen or antibody adsorption on the solid phase carrier ( polystyrene micro reaction plate ) surface, make an enzyme labeled antigen antibody reaction in solid phase surface, washing method in the liquid phase is washed free composition. The use of ELISA method with double antibody sandwich method and the indirect method, the former for the detection of macromolecular antigen, which is used for the determination of specific antibody ThisAntiß2GP1Abenzymelinkedimmunosorbentassayappliesatechniquecalledaquantitativesandwichimmunoassay.Themicrotiterplateprovidedinthiskithasbeenpre-coatedwithamonoclonalantibodyspecificforAntiß2GP1Ab.StandardsorsamplesarethenaddedtothemicrotiterplatewellsandAntiß2GP1Abifpresent,willbindtotheantibodypre-coatedwells.InordertoquantitativelydeterminetheamountofAntiß2GP1Abpresentinthesample,astandardizedpreparationofhorseradishperoxidase(HRP)-conjugatedpolyclonalantibody,specificforAntiß2GP1Abareaddedtoeachwellto“sandwich”theAntiß2GP1Abimmobilizedontheplate.Themicrotiterplateundergoesincubation,andthenthewellsarethoroughlywashedtoremoveallunboundcomponents.Next,AandBsubstratesolutionisaddedtoeachwell.Theenzyme(HRP)andsubstrateareallowedtoreactoverashortincubationperiod.OnlythosewellsthatcontainAntiß2GP1Abandenzyme-conjugatedantibodywillexhibitachangeincolour.Theenzyme-substratereactionisterminatedbytheadditionofasulphuricacidsolutionandthecolourchangeismeasuredspectrophotometricallyatawavelengthof450nm.
Related Articles -
Enzyme, linked, immunosorbent, assay,
|
Rate This Article  |
|
|
|
|
|
Do you Agree or Disagree? Have a Comment? POST IT!
| Reader Opinions |
|
|
|
|
|
|
|
|
|
| Author Login |
|
|
 |
Advertiser Login
ADVERTISE HERE NOW!
Limited Time $60 Offer!
90 Days-1.5 Million Views
|
|
TIM FAY
After 60-plus years of living, I am just trying to pass down some of the information that I have lea...more
|
|
|
|
|
GENE MYERS
Author of four books and two screenplays; frequent magazine contributor. I have four other books "in...more
|
|
|
|
|
ADRIAN JOELE
I have been involved in nutrition and weight management for over 12 years and I like to share my kn...more
|
|
|
|
|
LAURA JEEVES
At LeadGenerators, we specialise in content-led Online Marketing Strategies for our clients in the t...more
|
|
|
|
|
MICHAEL BRESCIANI
Rev Bresciani is the author of two Christian books. One book is an important and concisely written b...more
|
|
|
|
|
STEPHEN BYE
Steve Bye is currently a fiction writer, who published his first novel, ‘Looking Forward Through the...more
|
|
|
|
|
LEVAL AINAH
I am an internet marketer and also an educator. My goal is to help others who are looking to improve...more
|
|
|
|
|
PAUL PHILIPS
For more articles, blog messages & videos and a free e-book download go to www.NewParadigm.ws your p...more
|
|
|
|
|
ALEX BELSEY
I am the editor of QUAY Magazine, a B2B publication based in the South West of the UK. I am also the...more
|
|
|
|
