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Plant polysaccharide with a variety of important physiological functions, mainly induced immune substances, increase the immune function, inhibition of tumor growth and proliferation, such as lowering blood glucose and lipid-lowering effect. Recent studies show that Ginkgo biloba exocarp polysaccharides without removal of protein to kill tumor cells with increased formation of SOD, increased immune cell activity and enhance immune function and other effects [1 ~ 4], but on the removal of protein Ginkgo biloba activity of polysaccharide skin have not been reported. In this paper, Ginkgo biloba exocarp polysaccharides and the removal of protein polysaccharides on the immune function of mice immune to a period of Ginkgo biloba exocarp polysaccharides provide the scientific theoretical basis for research and development. Ginkgo 1 Equipment 1.1 Instrument Beckman DU640 UV spectrophotometer, Beckman Coulter, Inc.; Waterproof Electric Heating water bath, laboratory instrument factory in Hangzhou blue sky; BH-2 type OLYMPUS microscope, Olympus, Japan; Chua fluorescence microscope, Germany Chua company. 1.2 Materials and reagents of ginkgo biloba exocarp polysaccharides not deproteinized with GBEP-1 said that the polysaccharide content is 38.49%; removal of protein of ginkgo biloba exocarp polysaccharides with GBEP-2 that the polysaccharide content is 42.80%, both prepared in our laboratory; mouse (KM mouse clean), purchased in the Hayes Lake Laboratory Animal Co. Ltd., Certificate of Conformity: SCXK (Hu) 2002-0010 No., keeping the environment clean, qualified : SYXK (Soviet Union) 2002-0007; cyclophosphamide powder (CTX), Jiangsu Hengrui Medicine Co., Ltd.; injection, Chengdu, Hong Diao 9 Pharmacy Plants; normal saline (sodium chloride injection), a small business in Nanjing Pharmaceutical Factory; heparin injection, Wanbang Biochemical Pharmaceutical Co., Ltd.; Giemsa, Nanjing Jiancheng Biological Engineering Institute. 5% chicken red blood cells: blood from the commercial broiler about 10ml, anticoagulation with heparin saline, and then joined the injection of 1:1 0.9% NaCl solution with full mixing after washing, 3000r/min centrifugal 15min, discard supernatant, then washing the remaining red blood cells 2 to 3 times. Wash the red blood cells obtained after centrifugation 4ml, prepared with 0.9% NaCl solution into a 5% concentration, is now equipped with the [5 ~ 7]. 10% of the complement: take five different guinea pig nest blood, serum separated and combined with normal saline to match 10% concentration of serum saved backup. 2 Methods 2.1 mouse peritoneal macrophages experimental mice were taken 70, weight 18 ~ 22g, were randomly divided into 7 groups, n = 10, half and half, respectively, the normal group and the CTX model group (distilled water 20ml / kg), positive drug Injection Group (0.1ml / only), GBEP-1 large and small dose group (300,100 mg / kg), GBEP-2 large, low-dose group (300,100 mg / kg). In addition to positive drug group (intramuscular injection), each group of animals administered (ig) administration, dosage are 20ml/kg, ig1 times a day for consecutive 7d, the first 5 days than the normal control group, each animals injected CTX40mg/kg (0.1ml/10g), 6 days each repeat injection 1. After administration in the last 1 1h, each mouse was injected 5% chicken erythrocyte suspension 0.5ml / only, to the chicken red blood cell after 2h, the mice were killed. According to the literature [5,6] method of measuring phagocytosis of peritoneal macrophages, obtained by the following formula phagocytic percentage and phagocytic index. Phagocytosis percentage (%) = eat chicken red blood cell counts of 200 macrophages macrophages × 100% Phagocytic index = number of red blood cells were swallowed chicken macrophages 200 2.2 hemolysin antibody formation mouse experiment and take on the immune organs of mice seventy weight 18 ~ 22g, were randomly divided into 7 groups, n = 10, evenly divided. The normal group and CTX model group (distilled water 20ml/kg), positive drug Injection Group (0.1ml / only), GBEP-1 big, small dose group (300,100 mg / kg), GBEP-2 big and small dose group (300,100 mg / kg). In addition to positive drug group (intramuscular injection), each group of animals administered (ig) administration. Dosage are 20ml/kg, ig1 times a day for consecutive 12d, in the first 5 days after administration in all groups intraperitoneal injection of 5% chicken red blood cell suspension 0.2ml / only for immunization, in addition to 10d after the administration normal control group, each animal injected CTX40mg/kg (0.1ml/10g), the first 11 days of repeated injections 1, after the last administration 2h, mice orbital plexus blood, according to literature [5 7] Method of measuring hemolysin. De Jing in mice after the mice were sacrificed, called weight, and then whichever thymus and spleen weight, thymus index and spleen index calculated. We are high quality suppliers, our products such as Men Leather Messenger Bags Manufacturer , Ladies Leather Backpacks for oversee buyer. To know more, please visits Ladies Leather Hand Bags.
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